High-fidelity cas13
Web3 de mai. de 2024 · Therefore, we first fused dCas13X.1 with high-fidelity ADAR2dd (with E488Q/T375G, referred as ADAR2dd*) ... High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects Web23 de dez. de 2024 · Thus, high-fidelity Cas13 variants with minimal collateral effect are now available for targeted degradation of RNAs in basic research and therapeutic …
High-fidelity cas13
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Web27 de jul. de 2024 · Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13 has drawn broad interest to control gene expression and cell fate at the RNA level in general. Apart from RNA interference mediated by its endonuclease activity, the nuclease-deactivated form of Cas13 further provides a versatile RNA-guided RNA-targeting … Web🚨High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects. Improving Cas13 with rational design + protein engineering...
Web29 de nov. de 2024 · They discovered a family of CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half the size of other Cas proteins found in the so-called class 2 CRISPR-Cas systems. There are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). Web14 de ago. de 2024 · This work, entitled “High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects,” was published online in Nature Biotechnology on Aug. 11.
Web26 de mai. de 2024 · In addition, Cas13 can be used for targeted cancer therapy through degrading and manipulating cancer-associated transcripts with high efficiency and specificity. Moreover, Cas13-mediated programmable RNA manipulation tools offer invaluable opportunities for cancer research, identification of drug-resistance … Web18 de fev. de 2024 · Yang et al. demonstrated that RNA targeting of paternal Ube3a-ATS with a high-fidelity CRISPR-Cas13 (hfCas13x.1) system could restore Ube3a …
Web12 de ago. de 2024 · More information: Huawei Tong et al, High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects, Nature Biotechnology (2024). …
Web13 de jan. de 2024 · Hence high-fidelity Cas13 variants with minimal collateral effects have been developed, which could further elevate their utility for targeted degradation of RNAs … granite creek pools and spasWeb29 de ago. de 2024 · Off-target editing is also a critical issue in the Cas13 system, although Cas13 is already applied to the knockdown of transcripts, editing of base RNA and live … granite creek health \u0026 rehabilitation centerWeb17 de nov. de 2024 · Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 different Cas13 variants to identify … granite creek rd idaho cityWeb18 de fev. de 2024 · A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. Author links open overlay panel Jinhui Li 1 6, Zhixin Shen 1 6, Yajing Liu 1 6, Zixiang Yan 3 6, Yuanhua Liu 1 6, Xiang Lin 4, Junjie Tang 1, Ruimin Lv 1, Guannan Geng 1, Zhi-Qi Xiong 1, Changyang … granite creek ranch wyomingWeb17 de nov. de 2024 · Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease … granite creek rest areaWeb12 de ago. de 2024 · A recent study published in Nature Biotechnology revealed the development of high-fidelity Cas13 variants with markedly reduced collateral effects by mutagenesis and demonstrated the feasibility of hfCas13 for efficient on-target RNA degradation with almost no collateral damage in mammalian cells and animals. This work … granite creek rehab scottsdaleWeb11 de ago. de 2024 · High-fidelity Cas13 variants show similar RNA knockdown activity to wild-type Cas13 but no detectable collateral damage in transgenic mice or adeno … granite creek rehab